![]() Di Kang, Claudio Parolo, Sheng Sun, Nathan E.Electrostatic Force Triggering Elastic Condensation of Double-Stranded DNA for High-Performance One-Step Immunoassay. Chunyan Deng, Manman Zhang, Chunyan Liu, Honghua Deng, Yan Huang, Minghui Yang, Juan Xiang, Bin Ren.ACS Applied Materials & Interfaces 2018, 10 Application of Antibody-Powered Triplex-DNA Nanomachine to Electrochemiluminescence Biosensor for the Detection of Anti-Digoxigenin with Improved Sensitivity Versus Cycling Strand Displacement Reaction. Shan-Shan Yang, Ming-Hui Jiang, Ya-Qin Chai, Ruo Yuan, Ying Zhuo.Langmuir Analysis of the Binding Affinity and Kinetics for Surface Tethered Duplex DNA and a Ligand–Apoprotein Complex. Peptide-Mediated Electrochemical Steric Hindrance Assay for One-Step Detection of HIV Antibodies. Sadat Mahshid, Sara Mahshid, Alexis Vallée-Bélisle, Shana O. Journal of the American Chemical Society 2019, 141 A Nucleic Acid Nanostructure Built through On-Electrode Ligation for Electrochemical Detection of a Broad Range of Analytes. Subramaniam Somasundaram, Christopher J.Open Source Software for the Real-Time Control, Processing, and Visualization of High-Volume Electrochemical Data. Ploense, Martin Kurnik, Gabriel Ortega, Claudio Parolo, Tod E. Journal of the American Chemical Society 2021, 143 Strategies for Biomolecular Analysis and Continuous Physiological Monitoring. Amanda Clifford, Jagotamoy Das, Hanie Yousefi, Alam Mahmud, Jenise B.Orthogonal Multipotential Coding of all Four DNA Bases for Electrochemical Analysis and Sequencing. Carborane- or Metallacarborane-Linked Nucleotides for Redox Labeling. David Kodr, Cansu Pinar Yenice, Anna Simonova, Dijana Pavlović Saftić, Radek Pohl, Veronika Sýkorová, Mayreli Ortiz, Ludĕk Havran, Miroslav Fojta, Zbigniew J.An Allosteric Transcription Factor DNA-Binding Electrochemical Biosensor for Progesterone. Sabatelle, Sébastien Lecommandoux, Catherine M. Electrochemical DNA Scaffold-Based Sensing Platform for Multiple Modes of Protein Assay and a Keypad Lock System. Li Wang, Xu Wang, Zhiqiang Chen, Shufeng Liu.This article is cited by 114 publications. Sensors for both targets are sensitive (detection limits in the low nanomolar range), rapid (minutes), reusable, and selective enough to function directly in complex matrices including blood serum, soil, and foodstuffs. We generated streptavidin sensors exhibiting both signal-on (target binding increases the faradaic current) and signal-off behavior, of which only the signal-off approach was generalizable to the detection of antibodies. We optimized the scaffold using a biotin recognition element and streptavidin as a target to determine the variables that define sensor performance before then applying the approach to detection of anti-digoxigenin antibodies using the steroid as the recognition element. The binding of a protein to the small molecule recognition element alters the dynamics of the scaffold, increasing or decreasing the efficiency with which the redox tag collides with the electrode and thus altering the observed faradaic current. Our electrochemical approach utilizes a redox-tagged DNA signaling scaffold that is conjugated to a small molecule recognition element and is covalently attached to an interrogating electrode. Here we have demonstrated a general, sensitive, and selective approach for the detection of macromolecules that bind to specific small molecule recognition elements.
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